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Cold Spring Harb Protoc. 2014 Oct 1;2014(10):pdb.prot080028. doi: 10.1101/pdb.prot080028.

Purification of mitochondria by sucrose step density gradient centrifugation.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147-2408;
2
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520-8023; Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8023.

Abstract

Mitochondrial fractions isolated from tissue culture cells or tissue such as liver after differential centrifugation can be purified further by density gradient centrifugation. Here we describe the use of sucrose for this purpose because it is commonly used and inexpensive and the resulting mitochondria preparations are useful for many purposes.

PMID:
25275106
DOI:
10.1101/pdb.prot080028
[Indexed for MEDLINE]

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