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Cold Spring Harb Protoc. 2014 Oct 1;2014(10):pdb.prot080010. doi: 10.1101/pdb.prot080010.

Isolation of mitochondria from animal tissue.

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Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147-2408;
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520-8023; Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8023.


Rat or mouse liver is the most frequently used tissue for mitochondrial preparations because it is readily available, easy to homogenize, and replete with mitochondria. A motor-driven Teflon and glass Potter-Elvehjem homogenizer is the best choice for homogenizing liver, but if one is not available, this tissue is soft enough that a Dounce homogenizer with a loose (A) pestle can also be used. The yield and purity of the mitochondrial preparation will be influenced by the method and speed of preparation and the age and physiological condition of the animal.

[Indexed for MEDLINE]

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