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Oncotarget. 2014 Sep 30;5(18):8681-9.

Splice variant transcripts of the anterior gradient 2 gene as a marker of prostate cancer.

Author information

1
Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Eggenstein-Leopoldshafen, Germany.
2
Department of Environmental Health Sciences and Hospital Infection Control, Medical Center-University of Freiburg, Freiburg, Germany.
3
Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Eggenstein-Leopoldshafen, Germany. Heinrich Heine University, Institute of Toxicology, Düsseldorf, Germany.
4
Institute of Legal Medicine, Innsbruck Medical University, Innsbruck, Austria. Penn State Eberly College of Science, University Park, PA, USA.
5
Department of Urology, Medical Center-University of Freiburg, Freiburg, Germany.
6
Institute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany.
7
Department of Urology, Division of Experimental Urology, Innsbruck Medical University, Innsbruck, Austria.

Abstract

Anterior gradient 2 (AGR2) is a gene predominantly expressed in mucus-secreting tissues or in endocrine cells. Its expression is drastically increased in tumors including prostate cancer. Here we investigated whether AGR2 transcript levels can be used as a biomarker to detect prostate cancer (PCa). Using a PCR-based approach, we could show that in addition to the wild-type (AGRwt long and short) transcripts, five other AGR2 splice variants (SV) (referred to as AGR2 SV-C, -E, -F, -G and -H) were present in cancer cell lines. In tissue biopsies, SV-H and AGR2wt (short) distinguished between benign and PCa (p ≤ 0.05 n = 32). In urine exosomes, AGR2 SV-G and SV-H outperformed serum PSA. Receiver operating characteristic (ROC) curves showed the highest discriminatory power of SV-G and SV-H in predicting PCa. AGR2 SV-G and SV-H are potential diagnostic biomarkers for the non-invasive detection of PCa using urine exosomes.

PMID:
25237833
PMCID:
PMC4226713
DOI:
10.18632/oncotarget.2365
[Indexed for MEDLINE]
Free PMC Article

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