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Biosci Biotechnol Biochem. 2014;78(7):1203-5. doi: 10.1080/09168451.2014.915734. Epub 2014 May 29.

Functional expression of recombinant human macrophage β-glucan receptor dectin-1 using baculovirus-silkworm expression system.

Author information

1
a Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture , Meijo University, Tempaku-ku , Nagoya 468-8502 , Japan.

Abstract

Human macrophage dectin-1, a type II transmembrane β-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for binding activity. Recombinant dectin-1 specifically bound to some β-glucans, and the neck domain and N-linked oligosaccharide chains of human dectin-1 did not affect the ligand binding activity and specificity of the receptor.

KEYWORDS:

N-linked oligosaccharide chains; baculovirus–silkworm expression system; human dectin-1; neck domain; β-glucan receptor

PMID:
25229859
DOI:
10.1080/09168451.2014.915734
[Indexed for MEDLINE]

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