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J Gen Virol. 2014 Dec;95(Pt 12):2831-7. doi: 10.1099/vir.0.067884-0. Epub 2014 Aug 20.

A viral transcription factor exhibits antiviral RNA silencing suppression activity independent of its nuclear localization.

Author information

1
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center for Plant Biology, Box 7080, 75007 Uppsala, Sweden.
2
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119992 Moscow, Russia.
3
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center for Plant Biology, Box 7080, 75007 Uppsala, Sweden eugene.savenkov@slu.se.

Abstract

Viral suppressors of RNA silencing (VSRs) are critical for the success of virus infection and efficient accumulation of virus progeny. The chrysanthemum virus B p12 protein acts as a transcription factor to regulate cell size and proliferation favourable for virus infection. Here, we showed that the p12 protein suppressed RNA silencing and was able to complement a VSR-deficient unrelated virus. Moreover, p12 counter-silencing activity could be uncoupled from its function as a transcription factor in the nucleus. The altered p12 protein, which lacked a nuclear localization signal and was not imported into the nucleus, was able to suppress RNA silencing as efficiently as the native protein. The data revealed new aspects of p12 functioning and identified a novel role for this viral zinc-finger transcription factor. The results provided a general insight into one of the activities of the p12 protein, which appeared to possess more than one function.

PMID:
25143075
DOI:
10.1099/vir.0.067884-0
[Indexed for MEDLINE]

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