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Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12157-62. doi: 10.1073/pnas.1401712111. Epub 2014 Aug 5.

Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal.

Author information

1
Brighton and Sussex Medical School, Falmer BN19RY, United Kingdom;
2
Brighton and Sussex Medical School, Falmer BN19RY, United Kingdom;Department of Public Health and Infectious Diseases, Institut Pasteur, Cenci-Bolognetti Foundation, Sapienza University of Rome, 00185 Rome, Italy;
3
Institute for Medical Biochemistry and Molecular Biology, University Medicine, Ernst-Moritz-Arndt University, 17475 Greifswald, Germany;
4
School of Life Sciences, University of Sussex, Falmer BN19RY, United Kingdom;
5
Institute for Research and Innovation in Biomedicine, Rouen University, 76821 Rouen, France;Platform in Proteomics PISSARO, 76821 Mont-Saint-Aignan, France; and.
6
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305.
7
Brighton and Sussex Medical School, Falmer BN19RY, United Kingdom; p.ghezzi@bsms.ac.uk.

Abstract

The mechanism by which oxidative stress induces inflammation and vice versa is unclear but is of great importance, being apparently linked to many chronic inflammatory diseases. We show here that inflammatory stimuli induce release of oxidized peroxiredoxin-2 (PRDX2), a ubiquitous redox-active intracellular enzyme. Once released, the extracellular PRDX2 acts as a redox-dependent inflammatory mediator, triggering macrophages to produce and release TNF-α. The oxidative coupling of glutathione (GSH) to PRDX2 cysteine residues (i.e., protein glutathionylation) occurs before or during PRDX2 release, a process central to the regulation of immunity. We identified PRDX2 among the glutathionylated proteins released in vitro by LPS-stimulated macrophages using mass spectrometry proteomic methods. Consistent with being part of an inflammatory cascade, we find that PRDX2 then induces TNF-α release. Unlike classical inflammatory cytokines, PRDX2 release does not reflect LPS-mediated induction of mRNA or protein synthesis; instead, PRDX2 is constitutively present in macrophages, mainly in the reduced form, and is released in the oxidized form on LPS stimulation. Release of PRDX2 is also observed in human embryonic kidney cells treated with TNF-α. Importantly, the PRDX2 substrate thioredoxin (TRX) is also released along with PRDX2, enabling an oxidative cascade that can alter the -SH status of surface proteins and thereby facilitate activation via cytokine and Toll-like receptors. Thus, our findings suggest a model in which the release of PRDX2 and TRX from macrophages can modify the redox status of cell surface receptors and enable induction of inflammatory responses. This pathway warrants further exploration as a potential novel therapeutic target for chronic inflammatory diseases.

KEYWORDS:

cysteine oxidation; redox proteomics; thiol oxidation

PMID:
25097261
PMCID:
PMC4143057
DOI:
10.1073/pnas.1401712111
[Indexed for MEDLINE]
Free PMC Article

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