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Blood. 2014 Oct 23;124(17):2725-9. doi: 10.1182/blood-2014-02-559468. Epub 2014 Jul 25.

A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.

Author information

1
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
2
National Information Communications Technology Australia Victoria Research Laboratory, Department of Electrical and Electronic Engineering, School of Engineering.
3
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Computing and Information Systems, and Department of Mathematics and Statistics, The University of Melbourne, Australia; and.
4
Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, United Kingdom.

Abstract

In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

PMID:
25079356
DOI:
10.1182/blood-2014-02-559468
[Indexed for MEDLINE]
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