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Anal Chem. 2014 Aug 5;86(15):7987-95. doi: 10.1021/ac502103t. Epub 2014 Jul 17.

Targeted bioimaging and photodynamic therapy of cancer cells with an activatable red fluorescent bioprobe.

Author information

1
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratories of Organic Solids and Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Zhongguancun North First Street 2, Beijing 100190, China.

Abstract

A new red-emissive bioprobe TPE-red-2AP2H was developed by taking advantage of the unique emission feature of tetraphenylethylene and a cancer cell-specific peptide. By responding to the target protein and the acidic microenvironment of tumor cells, activated fluorescence bioimaging was achieved with high signal-to-noise ratio and without involving mutiple washing steps. Apart from targeting the membrane-anchored LAPTM4B proteins, TPE-red-2AP2H was successfully utilized to trace the intracellular movement of LAPTM4B protein. The generation of (1)O2 under visible light irradiation makes this bioprobe also promising for targeted-photodynamic therapy. By discriminating the expression level of the target protein, TPE-red-2AP2H can respond to the progression status of tumors with different photodynamic therapy effect.

PMID:
25002157
DOI:
10.1021/ac502103t
[Indexed for MEDLINE]

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