Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8221-4. doi: 10.1002/anie.201404323. Epub 2014 Jun 16.

Photodegradable hydrogels for capture, detection, and release of live cells.

Author information

1
Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA). dshin@ucdavis.edu.

Abstract

Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-α antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence "halo" after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.

KEYWORDS:

antibodies; hydrogels; microarrays; micropatterning; single-cell detection

PMID:
24931301
PMCID:
PMC4380505
DOI:
10.1002/anie.201404323
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center