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Science. 2014 Jun 6;344(6188):1160-1164. doi: 10.1126/science.1250778.

A mutually assured destruction mechanism attenuates light signaling in Arabidopsis.

Author information

1
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 and U.S. Department of Agriculture/Agriculture Research Service, Plant Gene Expression Center, Albany, CA 94710.
2
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143.
3
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305.
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Contributed equally

Abstract

After light-induced nuclear translocation, phytochrome photoreceptors interact with and induce rapid phosphorylation and degradation of basic helix-loop-helix transcription factors, such as PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), to regulate gene expression. Concomitantly, this interaction triggers feedback reduction of phytochrome B (phyB) levels. Light-induced phosphorylation of PIF3 is necessary for the degradation of both proteins. We report that this PIF3 phosphorylation induces, and is necessary for, recruitment of LRB [Light-Response Bric-a-Brack/Tramtrack/Broad (BTB)] E3 ubiquitin ligases to the PIF3-phyB complex. The recruited LRBs promote concurrent polyubiqutination and degradation of both PIF3 and phyB in vivo. These data reveal a linked signal-transmission and attenuation mechanism involving mutually assured destruction of the receptor and its immediate signaling partner.

PMID:
24904166
PMCID:
PMC4414656
DOI:
10.1126/science.1250778
[Indexed for MEDLINE]
Free PMC Article

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