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ACS Med Chem Lett. 2013 Sep 23;4(11):1091-6. doi: 10.1021/ml400304w. eCollection 2013 Nov 14.

Structures of human acetylcholinesterase bound to dihydrotanshinone I and territrem B show peripheral site flexibility.

Author information

1
New York Structural Biology Center, New York, New York 10027, United States.
2
Kitasato Institute for Life Sciences, Kitasato University , Tokyo 108-8641, Japan.
3
Departments of Neuroscience and Pharmacology, Mayo Clinic Florida , Jacksonville, Florida 32224, United States.

Abstract

Acetylcholinesterase is a critical enzyme that regulates neurotransmission by degrading the neurotransmitter acetylcholine in synapses of the nervous system. It is an important target for both therapeutic drugs that treat Alzheimer's disease and chemical warfare agents that cripple the nervous system and cause death through paralysis. The enzyme has both catalytic and peripheral sites to which inhibitors may bind. Structures of recombinant human acetylcholinesterase in complex with the natural product inhibitors dihydrotanshinone I and territrem B reveal dihydrotanshinone I binding that is specific to only the peripheral site and territrem B binding that spans both sites and distorts the protein backbone in the peripheral site. These inhibitors may function as important molecular templates for therapeutics used for treatment of disease and protection against nerve agents.

KEYWORDS:

Acetylcholinesterase; catalytic site; conformational change; dihydrotanshinone I; peripheral site; territrem B

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