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PLoS One. 2014 Jun 2;9(6):e97815. doi: 10.1371/journal.pone.0097815. eCollection 2014.

Chlorogenic acid decreases intestinal permeability and increases expression of intestinal tight junction proteins in weaned rats challenged with LPS.

Author information

1
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, China.
2
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.
3
Department of Statistics, Texas A&M University, College Station, Texas, United States of America.
4
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, Hubei, China; Department of Animal Science, Texas A&M University, College Station, Texas, United States of America.
5
Department of Animal Science, Texas A&M University, College Station, Texas, United States of America.
6
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.

Abstract

Chlorogenic acid, a natural phenolic acid present in fruits and plants, provides beneficial effects for human health. The objectives of this study were to investigate whether chlorogenic acid (CHA) could improve the intestinal barrier integrity for weaned rats with lipopolysaccharide (LPS) challenge. Thirty-two weaned male Sprague Dawley rats (21 ± 1 d of age; 62.26 ± 2.73 g) were selected and randomly allotted to four treatments, including weaned rat control, LPS-challenged and chlorogenic acid (CHA) supplemented group (orally 20 mg/kg and 50 mg/kg body). Dietary supplementation with CHA decreased (P<0.05) the concentrations of urea and albumin in the serum, compared to the LPS-challenged group. The levels of IFN-γ and TNF-α were lower (P<0.05) in the jejunal and colon of weaned rats receiving CHA supplementation, in comparison with the control group. CHA supplementation increased (P<0.05) villus height and the ratio of villus height to crypt depth in the jejunal and ileal mucosae under condictions of LPS challenge. CHA supplementation decreased (P<0.05) intestinal permeability, which was indicated by the ratio of lactulose to mannitol and serum DAO activity, when compared to weaned rats with LPS challenge. Immunohistochemical analysis of tight junction proteins revealed that ZO-1 and occludin protein abundances in the jejunum and colon were increased (P<0.05) by CHA supplementation. Additionally, results of immunoblot analysis revealed that the amount of occludin in the colon was also increased (P<0.05) in CHA-supplemented rats. In conclusion, CHA decreases intestinal permeability and increases intestinal expression of tight junction proteins in weaned rats challenged with LPS.

PMID:
24887396
PMCID:
PMC4041575
DOI:
10.1371/journal.pone.0097815
[Indexed for MEDLINE]
Free PMC Article

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