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Int Immunopharmacol. 2014 Jul;21(1):186-92. doi: 10.1016/j.intimp.2014.04.021. Epub 2014 May 9.

Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages.

Author information

1
Department of Life and Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea; Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, South Korea.
2
Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, South Korea.
3
R & D Center, CTOBIO Inc., Gyeonggi-do 445-913, South Korea.
4
Department of Life and Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea. Electronic address: dhkim@khu.ac.kr.

Abstract

The TNF-α expression-inhibitory effect of lactic acid bacteria (LAB) isolated from kimchi were measured in lipopolysaccharide (LPS)-stimulated peritoneal macrophages. Among the LAB evaluated, Lactobacillus plantarum CLP-0611 inhibited the IL-1β and IL-6 expression, as well as the NF-κB and AP1 activation in LPS-stimulated peritoneal macrophages. Therefore, we investigated its inhibitory effect on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. TNBS significantly induced colon shortening, as well as myeloperoxidase activity and macroscopic score. Oral administration of CLP-0611 significantly reduced TNBS-induced body weight loss, colon shortening, myeloperoxidase activity, IRAK-1 phosphorylation, NF-κB and MAP kinase (p38, ERK, JNK) activation, and iNOS and COX-2 expression. CLP-0611 also inhibited TNBS-induced expression of TNF-α, IL-1β, and IL-6. However, IL-10 expression was induced. CLP-0611 also induced the production of M2 macrophage markers (IL-10, arginase I and CD206). Based on these findings, CLP-0611 inhibits TLR-4-linked NF-κB and MAPK signaling pathways and polarizes M1 to M2-like macrophages, thus ameliorating colitis.

KEYWORDS:

Colitis; Lactobacillus plantarum CLP-0611; Macrophage; Polarization; TNF-α

PMID:
24815859
DOI:
10.1016/j.intimp.2014.04.021
[Indexed for MEDLINE]

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