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Platelets. 2015;26(2):177-85. doi: 10.3109/09537104.2014.891728. Epub 2014 Mar 28.

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads.

Author information

1
Department of Clinical and Experimental Medicine, Division of Transfusion Medicine, Faculty of Health Sciences, Linköping University , Linköping , Sweden .

Abstract

The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by aphaeresis technique (n = 7). Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage. Spontaneous and TRAP-6-induced adhesion to fibrinogen- and collagen-coated beads was analyzed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation. We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.

KEYWORDS:

Adhesion molecules; blood products; platelet glycoproteins; transfusion medicine

PMID:
24679340
DOI:
10.3109/09537104.2014.891728
[Indexed for MEDLINE]
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