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Oral Dis. 2015 Jan;21(1):e105-13. doi: 10.1111/odi.12238. Epub 2014 Apr 8.

Differential dendritic cell-mediated activation and functions of invariant NKT-cell subsets in oral cancer.

Author information

1
Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.

Abstract

OBJECTIVES:

Invariant natural killer T (iNKT) cells are unique subset of glycolipid-reactive T lymphocytes with potent antitumour characteristics. This study was planned to understand Th-like cytokine profiles of iNKT-cell subsets and modulation of their functions in response to glycolipid ligand and tumour cell lysate (TL).

SUBJECTS AND METHODS:

Cytokine profile of iNKT-cell subsets was evaluated from the peripheral blood of eight oral squamous cell carcinoma (OSCC) patients by flow cytometry and enzyme-linked immunosorbent assay (ELISA), while antitumour activity of iNKT cells was measured by methyl tetrazolium salt assay.

RESULTS:

CD4(+) (CD4(+) CD8(-)) iNKT subset from OSCC patients showed significant (P < 0.01) expansion and higher IL-4 production following activation with α-GalCer-pulsed DCs, while CD4(-) CD8(-) double negative (DN) and CD8(+) (CD4(-) CD8(+) iNKT subsets produced IFN-γ predominantly. iNKT cells showed significantly (P = 0.02) increased secretion of IFN-γ and enhanced cytotoxicity to KB and SCC-4 tumour cells in response to α-GalCer and TL-pulsed DCs.

CONCLUSION:

It appears that mutual balance/ratio of iNKT subsets may be important for their effector functions. Selectively expanded DN and CD8(+) iNKT cells with α-GalCer and TL may be a better candidate vaccine for iNKT-cell-based adoptive cancer immunotherapy.

KEYWORDS:

cytokine; cytotoxicity; dendritic cells; iNKT subsets; oral cancer; α-galactosylceramide

PMID:
24654917
DOI:
10.1111/odi.12238
[Indexed for MEDLINE]

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