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J Mycol Med. 2014 Jun;24(2):135-40. doi: 10.1016/j.mycmed.2014.01.062. Epub 2014 Feb 26.

Altered immune responses in patients with chronic mucocutaneous candidiasis.

Author information

1
Mycology research center, faculty of veterinary medicine, university of Tehran, Azadi street, Tehran, Iran. Electronic address: khosravi@ut.ac.ir.
2
Faculty of veterinary medicine, Amol university of special modern technologies, Amol, Iran.
3
Mycology research center, faculty of veterinary medicine, university of Tehran, Azadi street, Tehran, Iran.

Abstract

OBJECTIVE:

The purpose of this study was to investigate the lymphocyte transformation responses and cytokine secretion of peripheral blood mononuclear cells (PBMC) from patients with chronic mucocutaneous candidiasis (CMC).

METHODS:

Phytohaemagglutinin (PHA) mitogen and Candida albicans (C. albicans) antigen proliferation assays were performed by culturing PBMCs in RPMI 1640. The levels of interleukin (IL)-2, IL-10, IL-17 and interferon (IFN)-γ present in the supernatant of cultures were determined using enzyme-linked immunosorbent assay (ELISA).

RESULTS:

The results showed that most patients (92.3%) had a low proliferative response to C. albicans antigens and PHA. PBMCs from CMC patients produced lower levels of T (h)-1 cytokines IL-2 (78.5±59.8 pg/mL) and IFN-γ (115.1±43.3 pg/mL) in response to Candida antigens when compared to controls (Il-2: 177±103.6 pg/mL; IFN-γ: 330.3±21.6 pg/mL) (P<0.05). Conversely, we observed a partial enhancement of IL-10 in the patients (213.7±86.1 pg/mL). Production of IL-17 indicated no significant differences between patients and controls when stimulated by Candida antigens (21.5±8.6 pg/mL versus 32.4±12.2 pg/mL) and PHA (27.7±11.5 pg/mL versus 36.2±9.1 pg/mL), respectively.

CONCLUSION:

These findings suggest that Candida antigens trigger a Th2 instead of Th1 cytokine response in patients with CMC. For better understanding, further studies require on a larger number of patients into the future.

KEYWORDS:

Candidose chronique cutanéo-muqueuse; Cellular immunity; Chronic mucocutaneous candidiasis; Cytokines; Lymphocyte transformation; L’immunité cellulaire lymphocytaire; Transformation

PMID:
24582143
DOI:
10.1016/j.mycmed.2014.01.062
[Indexed for MEDLINE]

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