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J Cereb Blood Flow Metab. 2014 Apr;34(4):585-8. doi: 10.1038/jcbfm.2014.20. Epub 2014 Feb 5.

OAT3-mediated extrusion of the 99mTc-ECD metabolite in the mouse brain.

Author information

1
Molecular Probe Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
2
Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
3
Diagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.

Abstract

After administration of the (99m)Tc complex with N,N'-1,2-ethylenediylbis-L-cysteine diethyl ester ((99m)Tc-ECD), a brain perfusion imaging agent, the radioactive metabolite is trapped in primate brain, but not in mouse and rat. Here, we investigate the involvement of metabolite extrusion by organic anion transporter 3 (OAT3), which is highly expressed at the blood-brain barrier in mice, in this species difference. The efflux rate of radioactivity in the cerebrum of Oat3(-/-) mice at later phase was 20% of that of control mice. Thus, organic anion transporters in mouse brain would be involved in the low brain retention of radioactivity after (99m)Tc-ECD administration.

PMID:
24496177
PMCID:
PMC3982094
DOI:
10.1038/jcbfm.2014.20
[Indexed for MEDLINE]
Free PMC Article

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