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Cell Host Microbe. 2014 Jan 15;15(1):113-24. doi: 10.1016/j.chom.2013.12.009.

Chlamydia trachomatis-induced alterations in the host cell proteome are required for intracellular growth.

Author information

1
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.
2
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Brigham and Women's Hospital, Boston, MA 02215, USA; Howard Hughes Medical Institute.
3
Department of Microbiology and Molecular Genetics, Duke University Medical School, Durham, NC 22710, USA.
4
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: starnbach@hms.harvard.edu.

Abstract

Intracellular pathogens directly alter host cells in order to replicate and survive. While infection-induced changes in host transcription can be readily assessed, posttranscriptional alterations are more difficult to catalog. We applied the global protein stability (GPS) platform, which assesses protein stability based on relative changes in an adjoining fluorescent tag, to identify changes in the host proteome following infection with the obligate intracellular bacteria Chlamydia trachomatis. Our results indicate that C. trachomatis profoundly remodels the host proteome independently of changes in transcription. Additionally, C. trachomatis replication depends on a subset of altered proteins, such as Pin1 and Men1, that regulate the host transcription factor AP-1 controlling host inflammation, stress, and cell survival. Furthermore, AP-1-dependent transcription is activated during infection and required for efficient Chlamydia growth. In summary, this experimental approach revealed that C. trachomatis broadly alters host proteins and can be applied to examine host-pathogen interactions and develop host-based therapeutics.

PMID:
24439903
PMCID:
PMC3932326
DOI:
10.1016/j.chom.2013.12.009
[Indexed for MEDLINE]
Free PMC Article
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