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Protoplasma. 2014 Mar;251(2):307-16. doi: 10.1007/s00709-013-0602-z. Epub 2014 Jan 14.

Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy.

Author information

1
Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy (LCAM), Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Sciencepark 904, 1098 XH, Amsterdam, The Netherlands, m.a.hink@uva.nl.

Abstract

Originally developed for the field of physical chemistry, fluorescence fluctuation spectroscopy (FFS) has evolved to a family of methods to quantify concentrations, diffusion rates and interactions of fluorescently labelled molecules. The possibility to measure at the nanomolar concentration level and to combine these techniques with microscopy allow to study biological processes with high sensitivity in the living cell. In this review, the basic principles, challenges and recent developments of the most common FFS methods are being discussed and illustrated by intracellular applications.

PMID:
24420265
DOI:
10.1007/s00709-013-0602-z
[Indexed for MEDLINE]

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