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PLoS One. 2014 Jan 3;9(1):e83955. doi: 10.1371/journal.pone.0083955. eCollection 2014.

Human mammospheres secrete hormone-regulated active extracellular vesicles.

Author information

1
Metabolomics CIBERehd, Derio, Spain.
2
Cell Biology and Stem Cells Unit, CIBERehd, Derio, Spain.
3
Proteomics Platform, ProteoRed-ISCIII, CIBERehd, Derio, Spain.
4
Structural Biology, CIC bioGUNE, Derio, Spain.
5
Metabolomics CIBERehd, Derio, Spain ; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain.

Abstract

Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.

PMID:
24404144
PMCID:
PMC3880284
DOI:
10.1371/journal.pone.0083955
[Indexed for MEDLINE]
Free PMC Article

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