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Evid Based Complement Alternat Med. 2013;2013:594906. doi: 10.1155/2013/594906. Epub 2013 Dec 2.

Low-level laser stimulation on adipose-tissue-derived stem cell treatments for focal cerebral ischemia in rats.

Author information

1
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan ; Department of Physical Therapy, Hung Kuang University, Taichung 43302, Taiwan ; Department of Medicine, National Defense Medical Center, Taipei 114, Taiwan ; Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan.
2
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
3
Graduate Institute of Pharmaceutical Science and Technology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
4
Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.

Abstract

This study investigated the effects of large-area irradiation from a low-level laser on the proliferation and differentiation of i-ADSCs in neuronal cells. MTT assays indicated no significant difference between the amount of cells with (LS+) and without (LS-) laser treatment (P > 0.05). However, immunofluorescent staining and western blot analysis results indicated a significant increase in the neural stem-cell marker, nestin, following exposure to low-level laser irradiation (P < 0.05). Furthermore, stem cell implantation was applied to treat rats suffering from stroke. At 28 days posttreatment, the motor functions of the rats treated using i-ADSCs (LS+) did not differ greatly from those in the sham group and HE-stained brain tissue samples exhibited near-complete recovery with nearly no brain tissue damage. However, the motor functions of the rats treated using i-ADSCs (LS-) remained somewhat dysfunctional and tissue displayed necrotic scarring and voids. The western blot analysis also revealed significant expression of oligo-2 in the rats treated using i-ADSCs (LS+) as well as in the sham group (P < 0.05). The results demonstrated that low-level laser irradiation exerts a positive effect on the differentiation of i-ADSCs and can be employed to treat rats suffering from ischemic stroke to regain motor functions.

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