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Biotechniques. 2013 Nov;55(5):265-8. doi: 10.2144/000114101.

Single step BP/LR combined Gateway reactions.

Author information

1
Life Technologies, Carlsbad, CA.

Abstract

The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.

PMID:
24215642
DOI:
10.2144/000114101
[Indexed for MEDLINE]
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