Format

Send to

Choose Destination
Transgenic Res. 2014 Feb;23(1):177-85. doi: 10.1007/s11248-013-9764-x. Epub 2013 Nov 7.

Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.

Author information

1
The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK, er1@sanger.ac.uk.

Abstract

We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.

PMID:
24197666
PMCID:
PMC3890051
DOI:
10.1007/s11248-013-9764-x
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center