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Clin Cancer Res. 2014 Jan 1;20(1):140-150. doi: 10.1158/1078-0432.CCR-13-1434. Epub 2013 Oct 22.

Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation.

Author information

1
Institute for Regenerative Medicine at Scott & White, Molecular and Cellular Medicine Department, Texas A&M Health Science Center, Temple, Texas 76502, USA.
2
Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China.
3
Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
4
Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
5
Lutheran Medical Center, Brooklyn, NY 11220, USA.
6
Department of Radiation Oncology, Scott and White Hospital, Temple, Texas 76502, USA.
#
Contributed equally

Abstract

PURPOSE:

To examine the effects and mechanisms of transient activation of the Hedgehog pathway on rescuing radiotherapy-induced hyposalivation in survivors of head and neck cancer.

EXPERIMENTAL DESIGN:

Mouse salivary glands and cultured human salivary epithelial cells were irradiated by a single 15-Gy dose. The Hedgehog pathway was transiently activated in mouse salivary glands, by briefly overexpressing the Sonic hedgehog (Shh) transgene or administrating smoothened agonist, and in human salivary epithelial cells, by infecting with adenovirus encoding Gli1. The activity of Hedgehog signaling was examined by the expression of the Ptch1-lacZ reporter and endogenous Hedgehog target genes. The salivary flow rate was measured following pilocarpine stimulation. Salivary stem/progenitor cells (SSPC), parasympathetic innervation, and expression of related genes were examined by flow cytometry, salisphere assay, immunohistochemistry, quantitative reverse transcription PCR, Western blotting, and ELISA.

RESULTS:

Irradiation does not activate Hedgehog signaling in mouse salivary glands. Transient Shh overexpression activated the Hedgehog pathway in ductal epithelia and, after irradiation, rescued salivary function in male mice, which is related with preservation of functional SSPCs and parasympathetic innervation. The preservation of SSPCs was likely mediated by the rescue of signaling activities of the Bmi1 and Chrm1-HB-EGF pathways. The preservation of parasympathetic innervation was associated with the rescue of the expression of neurotrophic factors such as Bdnf and Nrtn. The expression of genes related with maintenance of SSPCs and parasympathetic innervation in female salivary glands and cultured human salivary epithelial cells was similarly affected by irradiation and transient Hedgehog activation.

CONCLUSIONS:

These findings suggest that transient activation of the Hedgehog pathway has the potential to restore salivary gland function after irradiation-induced dysfunction.

PMID:
24150232
PMCID:
PMC3951215
DOI:
10.1158/1078-0432.CCR-13-1434
[Indexed for MEDLINE]
Free PMC Article

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