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Chembiochem. 2013 Oct 11;14(15):1958-62. doi: 10.1002/cbic.201300453. Epub 2013 Sep 12.

Selective functionalization of the protein N terminus with N-myristoyl transferase for bioconjugation in cell lysate.

Author information

1
Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125 (USA) http://tirrell-lab.caltech.edu.

Abstract

A site to behold: Robust site-specific functionalization of engineered proteins is achieved with N-myristoyl transferase (NMT) in bacterial cells. NMT tolerates non-natural substrate proteins as well as reactive fatty acid tags, rendering it a powerful tool for protein conjugation applications, including the construction of protein microarrays from lysate.

KEYWORDS:

N-myristoyl transferase; chemoenzymatic; click chemistry; microarrays; protein engineering

PMID:
24030852
DOI:
10.1002/cbic.201300453
[Indexed for MEDLINE]

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