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Bioresour Technol. 2013 Oct;146:604-610. doi: 10.1016/j.biortech.2013.07.112. Epub 2013 Jul 30.

Biological abatement of cellulase inhibitors.

Author information

1
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Department of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
2
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
3
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
4
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States. Electronic address: ladisch@purdue.edu.

Abstract

Removal of enzyme inhibitors released during lignocellulose pretreatment is essential for economically feasible biofuel production. We tested bio-abatement to mitigate enzyme inhibitor effects observed in corn stover liquors after pretreatment with either dilute acid or liquid hot water at 10% (w/v) solids. Bio-abatement of liquors was followed by enzymatic hydrolysis of cellulose. To distinguish between inhibitor effects on enzymes and recalcitrance of the substrate, pretreated corn stover solids were removed and replaced with 1% (w/v) Solka Floc. Cellulose conversion in the presence of bio-abated liquors from dilute acid pretreatment was 8.6% (0.1x enzyme) and 16% (1x enzyme) higher than control (non-abated) samples. In the presence of bio-abated liquor from liquid hot water pretreated corn stover, 10% (0.1x enzyme) and 13% (1x enzyme) higher cellulose conversion was obtained compared to control. Bio-abatement yielded improved enzyme hydrolysis in the same range as that obtained using a chemical (overliming) method for mitigating inhibitors.

KEYWORDS:

Bio-abatement; Biomass liquor; Enzymatic hydrolysis; Inhibitors

PMID:
23973982
DOI:
10.1016/j.biortech.2013.07.112
[Indexed for MEDLINE]

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