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Biochemistry. 2013 Sep 10;52(36):6275-85. doi: 10.1021/bi4008676. Epub 2013 Aug 28.

Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus.

Author information

1
Department of Chemical and Biological Engineering and ‡Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York 12180, United States.

Abstract

Adeno-associated virus (AAV) is a key candidate in the development of gene therapy. In this work, we used surface plasmon resonance spectroscopy to study the interaction between AAV and heparin and other glycosaminoglycans (GAGs). Surface plasmon resonance results revealed that heparin binds to AAV with an extremely high affinity. Solution competition studies showed that binding of AAV to heparin is chain length-dependent. AAV prefers to bind full chain heparin. All sulfo groups (especially N-sulfo and 6-O-sulfo groups) on heparin are important for the AAV-heparin interaction. Higher levels of sulfo group substitution in GAGs enhance their binding affinities. Atomic force microscopy was also performed to image AAV-2 in a complex with heparin.

PMID:
23952613
PMCID:
PMC3859860
DOI:
10.1021/bi4008676
[Indexed for MEDLINE]
Free PMC Article

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