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Biomol NMR Assign. 2014 Oct;8(2):335-8. doi: 10.1007/s12104-013-9512-4. Epub 2013 Jul 29.

Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form.

Author information

1
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR, 97239-3098, USA.

Abstract

Nearly complete backbone resonance assignments for the 357 residue, 42 kDa enzyme arginine kinase in a transition state analogue (TSA) complex are presented. The TSA is a quaternary complex of arginine kinase, MgADP, arginine, and nitrate. About 93% (320 of 344) of the non-proline backbone amides were assigned using an enzyme enriched with (2)H, (13)C, and (15)N in combination with three enzyme samples prepared with a single (15)N-labeled amino acid (K, L, and R). The amide assignments will provide the foundation for investigating the dynamics of arginine kinase when in a TSA complex.

PMID:
23893440
PMCID:
PMC3906217
DOI:
10.1007/s12104-013-9512-4
[Indexed for MEDLINE]
Free PMC Article

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