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Open Biol. 2013 May 22;3(5):130053. doi: 10.1098/rsob.130053.

A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Author information

1
Cell Cycle Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.

Abstract

To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. A total of 513 genes have been identified as being required for cell cycle progression, 276 of which have not been previously described as cell cycle genes. Deletions of a further 333 genes lead to specific alterations in cell shape and another 524 genes result in generally misshapen cells. Here, we provide the first eukaryotic resource of gene deletions, which describes a near genome-wide set of genes required for the cell cycle and cell shape.

KEYWORDS:

cell cycle; cell shape; fission yeast; genome-wide gene deletion resource

PMID:
23697806
PMCID:
PMC3866870
DOI:
10.1098/rsob.130053
[Indexed for MEDLINE]
Free PMC Article

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