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Biosci Biotechnol Biochem. 2013;77(4):874-6. Epub 2013 Apr 7.

Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.

Author information

1
Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

Abstract

A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.

PMID:
23563551
DOI:
10.1271/bbb.120936
[Indexed for MEDLINE]
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