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Future Microbiol. 2013 Feb;8(2):257-69. doi: 10.2217/fmb.12.134.

Peptide targeting and imaging of damaged lung tissue in influenza-infected mice.

Author information

1
Interdisciplinary Research Group in Infectious Diseases, Singapore-Massachusetts Institute of Technology Alliance in Research & Technology, 1 CREATE Way, #03-12/13/14 Enterprise Wing, 138602, Singapore.

Abstract

AIM:

In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection.

MATERIALS & METHODS:

Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualized histologically.

RESULTS:

pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue.

CONCLUSION:

pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue.

PMID:
23374130
PMCID:
PMC3677567
DOI:
10.2217/fmb.12.134
[Indexed for MEDLINE]
Free PMC Article

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