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Biochem Biophys Res Commun. 2013 Feb 8;431(2):192-6. doi: 10.1016/j.bbrc.2012.12.136. Epub 2013 Jan 9.

Acetyl-keto-β-boswellic acid induces lipolysis in mature adipocytes.

Author information

1
Clinical Research Unit, Khoo Teck Puat Hospital, Singapore 768828, Republic of Singapore. liu.jianjun@alexandrahealth.com.sg

Abstract

Recently, it was reported that naturally occurring pentacyclic triterpenoids such as ursolic acid have anti-adiposity property. We studied if acetyl-keto-β-boswellic acid (AKBA), an established anti-inflammation and anti-cancer pentacyclic triterpenoid which has similar chemical structure to ursolic acid, may modulate adipocyte phenotype. 3T3-L1 murine adipocytes and human subcutaneous adipocytes were treated with AKBA in different concentrations in vitro. AKBA triggered significant lipolysis in 3T3-L1 adipocytes as shown by reduced neutral lipids in cytosol and increased free fatty acids in culture medium. Increased lipolysis by AKBA was accompanied by up-regulation of lipolytic enzymes, adipocyte triglyceride lipase (ATGL) and hormone sensitive lipase (HSL), and a decreased expression of lipid droplet stability regulator perilipin. In addition, AKBA treatment reduced phenotypic markers of mature adipocyte aP2, adiponectin and glut-4 in mature adipocytes. Further studies revealed that AKBA down-regulated PPAR-γ and C/EBP-α expression in a dose and temporal dependent manner in mature adipocytes. In human adipocytes, AKBA likewise mobilized lipolysis accompanied by down-regulation of PPAR-γ2 expression and loss of phenotypic markers of mature adipocytes.

PMID:
23313487
DOI:
10.1016/j.bbrc.2012.12.136
[Indexed for MEDLINE]

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