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Genes Dev. 2012 Nov 15;26(22):2477-82. doi: 10.1101/gad.202242.112.

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.

Author information

1
Cell Resource Center for Biomedical Research, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Miyagi, Japan.

Abstract

Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15(INK4b), a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

PMID:
23154982
PMCID:
PMC3505818
DOI:
10.1101/gad.202242.112
[Indexed for MEDLINE]
Free PMC Article

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