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Proteomics. 2013 Feb;13(3-4):558-67. doi: 10.1002/pmic.201200339. Epub 2012 Dec 27.

Proteomics for understanding miRNA biology.

Author information

1
Department of Biological Chemistry, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.

PMID:
23125164
PMCID:
PMC3715049
DOI:
10.1002/pmic.201200339
[Indexed for MEDLINE]
Free PMC Article

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