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Nat Immunol. 2012 Dec;13(12):1162-70. doi: 10.1038/ni.2446. Epub 2012 Oct 21.

Control of RelB during dendritic cell activation integrates canonical and noncanonical NF-κB pathways.

Author information

1
Signaling Systems Laboratory, Department of Chemistry and Biochemistry and San Diego Center for Systems Biology, University of California, San Diego, La Jolla, California, USA.

Abstract

The NF-κB protein RelB controls dendritic cell (DC) maturation and may be targeted therapeutically to manipulate T cell responses in disease. Here we report that RelB promoted DC activation not as the expected RelB-p52 effector of the noncanonical NF-κB pathway, but as a RelB-p50 dimer regulated by canonical IκBs, IκBα and IκBɛ. IκB control of RelB minimized spontaneous maturation but enabled rapid pathogen-responsive maturation. Computational modeling of the NF-κB signaling module identified control points of this unexpected cell type-specific regulation. Fibroblasts that we engineered accordingly showed DC-like RelB control. Canonical pathway control of RelB regulated pathogen-responsive gene expression programs. This work illustrates the potential utility of systems analyses in guiding the development of combination therapeutics for modulating DC-dependent T cell responses.

PMID:
23086447
PMCID:
PMC3634611
DOI:
10.1038/ni.2446
[Indexed for MEDLINE]
Free PMC Article

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