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J Med Primatol. 2012 Dec;41(6):407-11. doi: 10.1111/jmp.12024. Epub 2012 Oct 18.

Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta).

Author information

1
Department of Population Health and Reproduction, University of California-Davis, Davis, CA, USA. gmkarere@txbiomedgenetics.org

Abstract

BACKGROUND:

Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior.

METHODS:

We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data.

RESULTS:

Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective.

CONCLUSIONS:

Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

PMID:
23078595
PMCID:
PMC3492537
DOI:
10.1111/jmp.12024
[Indexed for MEDLINE]
Free PMC Article

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