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Methods Mol Biol. 2012;918:1-7. doi: 10.1007/978-1-61779-995-2_1.

Image-based analysis of light-grown seedling hypocotyls in Arabidopsis.

Author information

1
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. chory@salk.edu

Abstract

Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.

PMID:
22893281
DOI:
10.1007/978-1-61779-995-2_1
[Indexed for MEDLINE]

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