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Nat Methods. 2012 Sep;9(9):913-5. doi: 10.1038/nmeth.2137. Epub 2012 Aug 12.

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington, USA.

Abstract

We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.

PMID:
22886093
PMCID:
PMC3433648
DOI:
10.1038/nmeth.2137
[Indexed for MEDLINE]
Free PMC Article

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