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Sci Rep. 2012;2:428. doi: 10.1038/srep00428. Epub 2012 May 29.

siRNA off-target effects in genome-wide screens identify signaling pathway members.

Author information

1
National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD 20892, USA. eugen.buehler@nih.gov

Abstract

We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.

PMID:
22645644
PMCID:
PMC3361704
DOI:
10.1038/srep00428
[Indexed for MEDLINE]
Free PMC Article
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