Rapid-growth Marchantia paleacea calluses were induced on MSK2 medium through surface sterilisation of the capsula. Ten known compounds including two steroids (1-2), six bibenzyls (3, 5-9), a flavonoid (10), and a terpenoid (4) were isolated from these calluses. The allelopathic effect of the six bibenzyls was assessed in Arabidopsis thaliana. Results revealed that bibenzyls could inhibit seedling growth in a dose-dependent manner.