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J Microbiol Methods. 2012 Jan;88(1):172-4. doi: 10.1016/j.mimet.2011.10.009. Epub 2011 Oct 18.

High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type".

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1
Cork Institute of Technology, Cork, Ireland.

Abstract

In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.

PMID:
22033498
DOI:
10.1016/j.mimet.2011.10.009
[Indexed for MEDLINE]

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