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J Clin Microbiol. 2011 Oct;49(10):3647-8. doi: 10.1128/JCM.00389-11. Epub 2011 Aug 10.

Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis.

Author information

1
Department of Laboratory Medicine, Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.

Abstract

We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.

PMID:
21832023
PMCID:
PMC3187303
DOI:
10.1128/JCM.00389-11
[Indexed for MEDLINE]
Free PMC Article

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