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J Neurosci Methods. 2011 Sep 30;201(1):177-9. doi: 10.1016/j.jneumeth.2011.07.016. Epub 2011 Jul 23.

Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis.

Author information

1
Department of Neuroscience, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.

Abstract

Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions. A rapid dissection of the virus-expressing region is required for certain biochemical analyses. To improve the accuracy of the rapid dissection, we developed a method that employs a Bluestar flashlight in combination with barrier filter glasses to visualize the expression of EGFP lentivirus that has been microinjected into the nucleus accumbens. Processing of dissected tissue for EGFP immunoblotting further validated the approach.

PMID:
21816173
PMCID:
PMC3176337
DOI:
10.1016/j.jneumeth.2011.07.016
[Indexed for MEDLINE]
Free PMC Article

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