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Anal Chem. 2011 Aug 1;83(15):5989-95. doi: 10.1021/ac2009993. Epub 2011 Jun 30.

Fluorescent liposomes for differential interactions with glycosaminoglycans.

Author information

1
Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota 58108-6050, United States.

Abstract

We have successfully synthesized a lipid containing the pyranine dye as the hydrophilic headgroup. This lipid was incorporated into liposomes with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine as the major component. The resultant liposomes displayed differential modulations in fluorescence emission intensity in the presence of nanomolar concentrations of different glycosaminoglycans. Linear discriminant analysis of the fluorescence response data demonstrate that the liposomes are able to distinguish between different GAGs. In addition, we also demonstrate that the liposomes incorporating the pyranine lipid are able to distinguish between dilute serum from healthy individuals and serum containing elevated chondroitin sulfate (simulated serum from an Alzheimer's disease patient).

PMID:
21675793
PMCID:
PMC3146584
DOI:
10.1021/ac2009993
[Indexed for MEDLINE]
Free PMC Article

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