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Fertil Steril. 2011 Jul;96(1):203-7. doi: 10.1016/j.fertnstert.2011.05.016. Epub 2011 Jun 8.

Primate preimplantation embryo is a target for relaxin during early pregnancy.

Author information

1
California National Primate Research Center, University of California, Davis, CA 95616, USA. cavandevoort@ucdavis.edu

Abstract

OBJECTIVE:

To determine whether preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle.

DESIGN:

Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated, and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/mL) for 8 days.

SETTING:

Research laboratory.

ANIMAL(S):

Rhesus monkey.

INTERVENTION(S):

Controlled ovarian stimulation to obtain oocytes for in vitro-produced embryos that were cultured with or without human recombinant relaxin.

MAIN OUTCOME MEASURE(S):

Rate of blastocyst development, percentage of blastocysts, and inner cell mass/trophectoderm cell ratio were measured on day 8 of culture. The presence of relaxin receptor (RXFP1) messenger RNA in eight-cell embryos was observed by array hybridization.

RESULT(S):

RXFP1 receptor expression was localized to the inner cell mass of blastocysts, as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/trophectoderm cell ratio was unchanged with relaxin supplementation; however, the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos.

CONCLUSION(S):

These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.

PMID:
21645893
PMCID:
PMC3129389
DOI:
10.1016/j.fertnstert.2011.05.016
[Indexed for MEDLINE]
Free PMC Article

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