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Anaerobe. 2011 Dec;17(6):444-7. doi: 10.1016/j.anaerobe.2011.05.008. Epub 2011 May 23.

Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance.

Author information

1
Department of Laboratory Medicine, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic. dubska@mou.cz

Abstract

Based on experiments with 10 defined strains of Escherichia coli, we present a new method for bacterial phenotyping using SELDI-TOF mass spectrometry. Changes in bacterial protein profiles in the context of the time of cultivation and the antibiotic environment were minimal. Proteom subprofiling may further distinguish between strains with specific susceptibility to antimicrobials. Mass spec-based methods may become common in the future of bacterial pathogen identification in clinical microbiology diagnostics.

PMID:
21624485
DOI:
10.1016/j.anaerobe.2011.05.008
[Indexed for MEDLINE]

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