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J Clin Endocrinol Metab. 2011 Aug;96(8):E1298-302. doi: 10.1210/jc.2011-0278. Epub 2011 May 18.

Intact primary mitochondrial function in myotubes established from women with PCOS.

Author information

1
Department of Endocrinology, Odense University Hospital, Odense, Denmark. mette.eriksen@ouh.regionsyddanmark.dk

Abstract

CONTEXT:

Polycystic ovary syndrome (PCOS) affects 5-8% of fertile women and is often accompanied by insulin resistance, leading to increased risk of developing type 2 diabetes. Skeletal muscle from insulin-resistant PCOS subjects display reduced expression of nuclear encoded genes involved in mitochondrial oxidative metabolism.

OBJECTIVE:

We aimed to investigate whether there was a primary mitochondrial dysfunction or difference in mitochondria content that might contribute to the in vivo detected insulin resistance.

DESIGN:

The ATP synthesis with and without ATP use and the mitochondrial mass was determined in mitochondria isolated from myotubes established from PCOS subjects and control subjects.

PATIENTS:

Myotubes were established from eight insulin-resistant PCOS subjects (verified by euglycemic hyperinsulinemic clamp) and eight healthy weight- and age-matched controls.

RESULTS:

Mitochondrial mass and measurable mitochondrial ATP synthesis, with and without ATP use, were not different between PCOS subjects and control subjects.

CONCLUSION:

We found no evidence for a primary impaired mitochondrial function or content in myotubes established from PCOS subjects, and our results suggest that reduced expression of oxidative genes in PCOS subjects is an adaptive trait.

PMID:
21593108
DOI:
10.1210/jc.2011-0278
[Indexed for MEDLINE]

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