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Cell Commun Signal. 2011 Apr 11;9(1):8. doi: 10.1186/1478-811X-9-8.

A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells.

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1
Center of Regenerative Medicine in Barcelona, Dr, Aiguader 88, 08003 Barcelona, Spain. belmonte@salk.edu.

Abstract

Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

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