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J Biochem. 2011 Feb;149(2):219-27. doi: 10.1093/jb/mvq138. Epub 2010 Nov 26.

Comparison of recombinant protein expression in a baculovirus system in insect cells (Sf9) and silkworm.

Author information

1
Katakura Industries Co. Ltd, Research Institute of Biological Science, Sayama, Saitama, Japan.

Abstract

Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid baculovirus was unsuccessful for one protein, and two proteins were not expressed. A similar pattern of expression was seen in both silkworm and Sf9 cells, with double and multiple bands found in immunoblotting of the precipitate of both hosts. Degraded proteins were seen only in the silkworm system (particularly in the larvae). Production was more efficient in silkworms; a single silkworm produced about 70 times more protein than 10(6) Sf9 cells in 2 ml of culture medium.

PMID:
21113054
DOI:
10.1093/jb/mvq138
[Indexed for MEDLINE]

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