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J Biol Chem. 2011 Jan 14;286(2):1389-99. doi: 10.1074/jbc.M110.184077. Epub 2010 Nov 8.

Intracellular regions of the Eag potassium channel play a critical role in generation of voltage-dependent currents.

Author information

1
Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, Florida 32306, USA. liyong68@shsmu.edu.cn

Abstract

Folding, assembly, and trafficking of ion channels are tightly controlled processes and are important for biological functions relevant to health and disease. Here, we report that functional expression of the Eag channel is temperature-sensitive by a mechanism that is independent of trafficking or surface targeting of the channel protein. Eag channels in cells grown at 37 °C exhibit voltage-evoked gating charge movements but fail to conduct K(+) ions. By mutagenesis and chimeric channel studies, we show that the N- and C-terminal regions are involved in controlling a step after movement of the voltage sensor, as well as in regulating biophysical properties of the Eag channel. Synthesis and assembly of Eag at high temperature disrupt the ability of these domains to carry out their function. These results suggest an important role of the intracellular regions in the generation of Eag currents.

PMID:
21059657
PMCID:
PMC3020747
DOI:
10.1074/jbc.M110.184077
[Indexed for MEDLINE]
Free PMC Article

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